Food pathogens identified in 8-24 hour
The AOAC Research Institute has granted Performance Tested Method status to Bio-Rad Laboratories' iQ-CheckTM test kits. The iQ-Check family of kits is based on automated real-time polymerase chain reaction (RTi-PCR) amplification and detection. Currently, kits are available for Listeria spp., Listeria monocytogenes, Salmonella, and E. coli O157:H7, all of which are approved. All tests can be run at the same time in the same reaction plate. Since the reaction occurs in closed PCR tubes, the chance for cross-contamination is limited. An internal amplification control is performed in each well to verify the validity of the PCR and confirm a negative result.
Two instrument platforms are available, to meet every users needs. The 96-well instrument is suitable for high throughput analysis, with the ability to run 4 instruments from a single computer at the same time. For lower volume users, we offer a 48-well instrument, also with the ability to run 4 instruments from a single computer at the same time. Since Bio-Rad manufacturers both of these instruments, we provide complete instrument and kit technical support.
iQ-Check E. coli O157:H7 is validated with a non-specific 8-24 hour enrichment in Buffered Peptone Water. Modified EC broth (as per USDA MLG) and EHEC Enrichment broth (as per FDA BAM) were also validated for use with shortened enrichment times. iQ-Check Salmonella II requires a single 21 ± 1 hour enrichment in nonselective Buffered Peptone Water, with no selective enrichment step. iQ-Check Listeria spp. and iQ-Check Listeria monocytogenes II are validated with a 25 ± 1 hour enrichment in Listeria Special Broth (LSB), a 24 hour time saving over the reference method. LSB is an enrichment media specially formulated to meet the growth requirements of Listeria while inhibiting competitor organisms.
Aporte: Guillermo Figueroa