martes, 24 de noviembre de 2009

Salmonella Detection in Peanut Butter: Validation of a Real-Time PCR Method

Salmonella Detection Kit was validated for samples containing peanut butter in response to a Salmonella outbreak in the USA caused by contaminated peanut butter with more than 700 infected persons, 9 deaths and a recall of more than 3,900 food products.

BIOTECON Diagnostics and Merck KGaA participated in an AOAC Emergency Response Validation with their real-time PCR kit for the rapid detection of Salmonella in food samples containing peanut butter.

Two validation studies were carried out with peanut butter spiked with different levels of Salmonella serovar Typhimurium. This technical paper shows that the performance of the foodproof® Salmonella Detection Kit in combination with the foodproof® ShortPrep I Kit for DNA extraction is equivalent to the FDA BAM reference method.