martes, 3 de abril de 2012

Gold nanoparticles help to faster Salmonella detection in produce

Gold nanoparticles acts in the outer membrane of the Salmonella, killing the pathogen
The test for lettuce requires just a tiny sample of lettuce leaf, also It doesn’t take a trained laboratory technician to perform the test or read the results. If the color changes from pink to bluish, that signals the presence of Salmonella. The test is suitable for use in farm fields and in remote areas of the developing world. We believe it may have enormous potential for rapid, on-site pathogen detection to avoid the distribution of contaminated foods.
 To find the bacteria faster, researchers at Jackson State University enlisted gold nanoparticles that are 1 million times smaller than an ant. They attached antibodies similar to those that help the immune system find and fight infections with Salmonella, to the nanoparticles. Viewed under a powerful microscope, the gold nanoparticles look somewhat like individual pieces of popcorn.
When these antibodies encounter Salmonella pathogen, they attach to the outer surface of the bacteria, carrying along their cargo of gold popcorn-shaped nanoparticles. The test, with its pink-to-blue color change, detects those gold nanoparticle-antibody-Salmonella complexes.
 The approach also has potential for killing MDR Salmonella, Ray said. When you shine the right wavelength of light into contaminated water, for instance, the gold nanoparticles absorb that light and heat up. Those hot particles burn through the outer membrane of the Salmonella, killing the pathogen.
 The researchers first developed the popcorn-shaped particles to find and fight cancer. The shape was chosen because it boosts the signal for detection using something called Raman spectroscopy, which looks at the light given off after atoms or molecules absorb energy. According to the investigators this detection method is useful in other applications of the particles. In science this is called the lightning rod effect, describing how the splayed “tips” of the popcorn shape enhance the signal and make it easier to see. The group has also used the nanoparticles to detect other microbes, like E. coli.
 Despite gold’s stature as a precious and very costly metal, only tiny amounts are needed. About $90 worth of gold is enough to make gallons of the solution containing the nanoparticles. And only a few drops of the solution are needed seek out Salmonella pathogen.
 The new technology can be commercialized, and a patent is pending. With concerns about the potential health and environmental effects of many kinds of nanoparticles, the team is investigating the effects of gold nanoparticles remaining in purified water, for instance. So far, they have found no short-term toxicity and will be checking on any potential long-term toxicity.
 Funding for this research at JSU comes in part from the National Science Foundation.

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