jueves, 8 de agosto de 2013
3M Molecular Detection Assay for Salmonella is adopted as an official method by AOAC international
The system is able to provide real-time results in as early as 15 minutes
The assays use loop-mediated isothermal amplification with high specificity and sensitivity, combined with bioluminescence to detect the amplification. The method uses multiple primers to recognize distinct regions of the genome and a DNA polymerase to provide continuous and rapid amplification.
Foods targeted: The matrices in the validation are pasteurized liquid whole egg, raw ground beef, cooked breaded chicken, raw shrimp, bagged spinach and wet pet food. There is a plan to expand the validation scope with additional matrices, in the case of the 3M Molecular Detection Assay - Salmonella and other Molecular Detection assays.
OMA validation is AOAC internationally recognized program for chemical, microbial and molecular biological testing methods. Consisting of a joint validation in multiple labs and reviewed by an expert panel, it ascribes a given method as an official method of analysis.
OMA expansion of PTM:The OMA builds on the Performance Tested Method (PTM), which compares new and alternative testing methods with standard reference methods for each food and environmental matrix. PTM tests also include inclusivity and exclusivity strains, as well as a series of tests challenging the robustness of the method’s instrumentation.
In the assay, pyrophosphate ions, generated by amplification of the targeted DNA, and a substrate are enzymatically converted into Adenosine triphosphate (ATP) by ATP-Sulfurylase. ATP reacts with luciferase to produce light, which is then detected by the 3M Molecular Detection Instrument,08/08/13 3M MDS Salmonella Kit with Media indicating the presence of target organism DNA.
Both amplification and detection occur simultaneously and continuously during the exponential phase providing real time results and a short run time. The system is able to provide real-time results in as early as 15 minutes, following an overnight enrichment and simple assay preparation protocol. Negative results are reported at the end of the 75-min instrument run.