jueves, 6 de junio de 2013
Invisible Sentinel's Veriflow Granted PTM Status for the Rapid Detection of Campylobacter Species
The assay utilizes a PCR detection method coupled with a rapid, visual, flow-based assay that develops in 3 minutes post-sample addition and requires only 24 hours
Veriflow CA is the first ultrasensitive, and user friendly molecular diagnostics being developed by Invisble Sentinel. This technology combines the sensitivity of molecular amplification with the simplicity of flow-based diagnotics, it also minimizes sample preparation, speeds time-to-results, and provides easy-to-interpret data for the end user. The Veriflow CA system utilizes a simplified enrichment procedure that does not require the use of a microaerobic chamber.
Due to specialized growth requirements and complex culture-based detection methods, Campylobacter is difficult to detect and current screening methods can take up to a week to produce results. Ins contrast, Veriflow CA delivers accurate, molecular-based results while minimizing the time-to-results associated with conventional detection methods.
Principle of the Test
Veriflow CA is able to detect most commonly ocurring Campylobacter species: C. jejuni and C. coli. The assay utilizes a PCR detection method coupled with a rapid, visual, flow-based assay that develops in 3 minutes post-sample addition and requires only 24 hours of nonspecialized culturing for maxium sensitivity. Veriflow eliminate the need for microaerobic chambers, gel electrophoresis, and does not require computerized data analysis.
Method Comparison Study
A method comparison study was performed to compare Veriflow CA to the USDA/FSIS MLG 41.01 reference method for the detection of naurraly ocurring C. jejuni and C. coli in chicken carcass rinsates.
In this study 40 chicken carcasses were analyzed per method. Following the Veriflow CA method, 24 out of 40 chickens were found to be positive after 24 hours of nonspecialized incubation. Each positive sample was confirmed as Campylobacter using confirmation procedures outlined in the USDA/FSIS MLG method. Relative sensitivity was calculated at 100% The independent reference method procedure detect 20 out of 40 chickens after a 96-hour incubation followed by an additional 24 hours of confirmatory analysis.
Veriflow CA represents a new class of molecular diagnostics that offers significant performance advantages over current detection methods. This study demonstrated the superior detection capabilities of Veriflow CA as compared to the reference method.
Overall, this study showed Veriflow CA to be an effective new diagnostics technology capable of delivering accurate, rapid, and easy-to-interpret results to the end-user for the detection of C. jejuni and C. coli from chicken carcass rinsates.